ACL VLAN question

Sorry if this is the wrong to post this.

I have a layer3 switch with 2 VLANs (VLAN 10 and VLAN 20)

I want to only allow PC1 (vlan 10) to connect to PC2 (vlan 20) on port 8000.

This is only communication I want between VLAN10 and VLAN20. So I plan to apply an ACL on VLAN 10 to permit this.

My question if I apply another ACL on VLAN 20 to block all traffic into VLAN10, will PC1 still be able to connect to PC2 on port 8000?

Thanks